17/03/2016
International workshop on ‘Surveillance and diagnostics for
equine influenza’ for delegates from SAARC nations under
OIE twinning project on Equine Influenza
16-25 Feb, 2016
An International workshop on ‘Surveillance and diagnostics for
equine influenza’ for delegates from SAARC nations was organized under
‘OIE twinning project on Equine influenza’ at National Research Centre on
Equines, India from 16-25 Feb, 2016. The workshop was attended by 16
delegates: one each from five SAARC countries viz. Sri Lanka, Bangladesh,
Bhutan, Nepal, Afghanistan and 11 delegates from India including Jammu
and Kashmir (1), Himachal Pradesh (1), Punjab (1), Rajasthan (1),
Uttarakhand (2), Uttar pradesh (2), Gujarat (1), Maharashtra (1) and
Animal Quarantine Station, Mumbai (1).
Collaborating institutes
National Research Centre on Equines,
Hisar, Haryana, India
&
Animal Health Trust,
Newmarket, UK
The training was provided by Experts from NRCE, India (Dr Nitin
Virmani, Dr B.C.Bera) and Animal Health Trust, UK (Dr Debra Elton and
Elizabeth Medcalf). The training was strictly ‘Hands On’ training with
emphasis on learning on doing.
During the inaugural session Dr. B.N. Tripathi, Director, NRCE gave an
overview of the research and activities of the institute and Indian scenario
of Equine influenza. A training manual was released during the
inauguration of the workshop.
Participants were trained in various techniques required for isolation
of equine influenza viruses in embryonated eggs/ MDCK cells, biosafety
and biosecurity, virus titration through haemgglutination,
Haemagglutination inhibition assay, Antigenic characterization of EIV by
HI assay using ferret antisera, Monoclonal antibody based Sandwich ELISA
for EI Antigen detection, Single step RT-PCR for diagnosis of EI infection,
Two step subtyping RT-PCR for HA/NA genes of EIV (H3N8), TaqMan Probe
based qRT-PCR for diagnosis of EI infection.
The delegates were also taken for one day Field visit to
Hanumangarh – to have real time feeling of our extension work, liaisoning
with stakeholders, sample collection and developing a network across the
country. This part was equally important for the participants as they could
actually visualize and practically collect samples from horses in the field.
Further the participants were detailed through lectures on various aspects
of equine influenza virus, transmission patterns, Quality control & GLP in the
laboratory, Control & management of EI - Biorisk & biohazard and
Vaccine strategies for equine influenza at the face of outbreak.